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Anderson, A.C.* ; Rothballer, M. ; Altenburger, M.J.* ; Woelber, J.P.* ; Karygianni, L.* ; Lagkouvardos, I.* ; Hellwig, E.* ; Al-Ahmad, A.*

In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption.

Sci. Rep. 8:14202 (2018)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
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Caries is associated with shifts of microbiota in dental biofilms and primarily driven by frequent sucrose consumption. Data on environmentally induced in vivo microbiota shifts are scarce therefore we investigated the influence of frequent sucrose consumption on the oral biofilm. Splint systems containing enamel slabs were worn for 3 x 7 days with 7-day intervals to obtain oral biofilm samples. After a three-month dietary change of sucking 10 g of sucrose per day in addition to the regular diet, biofilm was obtained again at the end of the second phase. The microbiota was analysed using Illumina MiSeq amplicon sequencing (v1-v2 region). In addition, roughness of the enamel surface was measured with laser scanning microscopy. The sucrose phase resulted in significant differences in beta-diversity and significantly decreased species richness. It was marked by a significant increase in abundance of streptococci, specifically Streptococcus gordonii, Streptococcus parasanguinis and Streptococcus sanguinis. Enamel surface roughness began to increase, reflecting initial impairment of dental enamel surface. The results showed that frequent sucrose consumption provoked compositional changes in the microbiota, leading to an increase of non-mutans streptococci, hence supporting the extended ecological plaque hypothesis and emphasizing the synergy of multiple bacterial species in the development of caries.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Early-childhood Caries; Dental-caries; Enamel Demineralization; Molecular Analysis; Fluoride Toothpaste; Permanent Teeth; Young-adults; Health; Sugars; Bacteria
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 8, Issue: 1, Pages: , Article Number: 14202 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Network Biology (INET)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Environmental Sciences
PSP Element(s) G-506400-001
DOI 10.1038/s41598-018-32544-6
WOS ID WOS:000445276000046
Scopus ID 85053717887
PubMed ID 30242260
Erfassungsdatum 2018-10-01
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