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Adiponectin affects the mechanical responses in strips from the mouse gastric fundus.
World J. Gastroenterol. 24, 4028-4035 (2018)
AIMTo investigate whether the adipocytes derived hormone adiponectin (ADPN) affects the mechanical responses in strips from the mouse gastric fundus.METHODSFor functional experiments, gastric strips from the fundal region were cut in the direction of the longitudinal muscle layer and placed in organ baths containing Krebs-Henseleit solution. Mechanical responses were recorded via force-displacement transducers, which were coupled to a polygraph for continuous recording of isometric tension. Electrical field stimulation (EFS) was applied via two platinum wire rings through which the preparation was threaded. The effects of ADPN were investigated on the neurally-induced contractile and relaxant responses elicited by EFS. The expression of ADPN receptors, Adipo-R1 and Adipo-R2, was also evaluated by touchdown-PCR analysis.RESULTSIn the functional experiments, EFS (4-16 Hz) elicited tetrodotoxin (TTX)-sensitive contractile responses. Addition of ADPN to the bath medium caused a reduction in amplitude of the neurally-induced contractile responses (P < 0.05). The effects of ADPN were no longer observed in the presence of the nitric oxide (NO) synthesis inhibitor L-N-G-nitro arginine (L-NNA) (P > 0.05). The direct smooth muscle response to methacholine was not influenced by ADPN (P > 0.05). In carbachol precontracted strips and in the presence of guanethidine, EFS induced relaxant responses. Addition of ADPN to the bath medium, other than causing a slight and progressive decay of the basal tension, increased the amplitude of the neurally-induced relaxant responses (P < 0.05). Touchdown-PCR analysis revealed the expression of both Adipo-R1 and Adipo-R2 in the gastric fundus.CONCLUSIONThe results indicate for the first time that ADPN is able to influence the mechanical responses in strips from the mouse gastric fundus.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Adiponectin ; Adiponectin Receptors ; Gastric Motility ; Nitric Oxide ; Non-adrenergic ; Non-cholinergic Neurotransmission; Nitric-oxide; Food-intake; Metabolic Homeostasis; Smooth-muscle; Body-weight; Mice; Leptin; Brain; Neurotransmission; Adipokines
ISSN (print) / ISBN
1007-9327
e-ISSN
2219-2840
Quellenangaben
Volume: 24,
Issue: 35,
Pages: 4028-4035
Publisher
WJG Press
Publishing Place
8226 Regency Dr, Pleasanton, Ca 94588 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Cancer (IDC)