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Kaczmarek-Hajek, K.* ; Zhang, J.* ; Kopp, R.* ; Grosche, A.* ; Rissiek, B.* ; Saul, A.* ; Bruzzone, S.* ; Engel, T.* ; Jooss, T.* ; Krautloher, A.* ; Schuster, S.* ; Magnus, T.* ; Stadelmann, C.* ; Sirko, S. ; Koch-Nolte, F.* ; Eulenburg, V.* ; Nicke, A.*

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody.

eLife 7:e36217 (2018)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The P2X7 channel is involved in the pathogenesis of various CNS diseases. An increasing number of studies suggest its presence in neurons where its putative functions remain controversial for more than a decade. To resolve this issue and to provide a model for analysis of P2X7 functions, we generated P2X7 BAC transgenic mice that allow visualization of functional EGFP-tagged P2X7 receptors in vivo. Extensive characterization of these mice revealed dominant P2X7-EGFP protein expression in microglia, Bergmann glia, and oligodendrocytes, but not in neurons. These findings were further validated by microglia- and oligodendrocyte-specific P2X7 deletion and a novel P2X7-specific nanobody. In addition to the first quantitative analysis of P2X7 protein expression in the CNS, we show potential consequences of its overexpression in ischemic retina and post-traumatic cerebral cortex grey matter. This novel mouse model overcomes previous limitations in P2X7 research and will help to determine its physiological roles and contribution to diseases.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Receptor-messenger-rna; Muller Glial-cells; P2x(7) Receptors; Cerebral-cortex; Status Epilepticus; Cmt1a Neuropathy; Gene-expression; Mice; Retina; Pore
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 2050-084X
e-ISSN 2050-084X
Journal eLife
Quellenangaben Volume: 7, Issue: , Pages: , Article Number: e36217 Supplement: ,
Publisher eLife Sciences Publications
Publishing Place Sheraton House, Castle Park, Cambridge, Cb3 0ax, England
Reviewing status Peer reviewed
Institute(s) Institute of Stem Cell Research (ISF)
POF-Topic(s) 30204 - Cell Programming and Repair
Research field(s) Stem Cell and Neuroscience
PSP Element(s) G-500800-001
DOI 10.7554/eLife.36217
WOS ID WOS:000444529700001
Scopus ID 85054088228
PubMed ID 30074479
Erfassungsdatum 2018-10-01
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