Home
Deutsch
Advanced Search
Browse by ...
Publication overview
Contact persons
Help
DOI
PMC
 |
|
Open Access Green as soon as Postprint is submitted to ZB.
Determination of enrichment factors for modified RNA in MeRIP experiments.
Methods 156, 102-109 (2019)
In the growing field of RNA modification, precipitation techniques using antibodies play an important role. However, little is known about their specificities and protocols are missing to assess their effectiveness. Here we present a method to assess enrichment factors after MeRIP-type pulldown experiments, here exemplified with a commercial antibody against N6-methyladenosine (m(6)A). Testing different pulldown and elution conditions, we measure enrichment factors of 4-5 using m(6)A-containing mRNAs against an unmodified control of identical sequence. Both types of mRNA carry P-32 labels at different nucleotides, allowing their relative quantification in a mixture after digestion to nucleotides, separation by TLC and quantitative phosphorimaging of the labels.
Altmetric
Additional Metrics?
Edit extra informations
Login
Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Merip ; Antibody ; Rna Modification ; M(6)a/n6-methyladenosine ; Tlc; Messenger-rna; Methylation; N-6-methyladenosine; Reveals; Translation; M6a
ISSN (print) / ISBN
1046-2023
e-ISSN
1095-9130
Journal
Methods
Quellenangaben
Volume: 156,
Pages: 102-109
Publisher
Elsevier
Publishing Place
Amsterdam [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CF Monoclonal Antibodies (CF-MAB)