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Critical role of the UBL domain in stimulating the E3 ubiquitin ligase activity of UHRF1 toward chromatin.
Mol. Cell 72, 739-752 (2018)
The RING E3 ubiquitin ligase UHRF1 controls DNA methylation through its ability to target the maintenance DNA methyltransferase DNMT1 to newly replicated chromatin. DNMT1 recruitment relies on ubiquitylation of histone H3 by UHRF1; however, how UHRF1 deposits ubiquitin onto the histone is unknown. Here, we demonstrate that the ubiquitin-like domain (UBL) of UHRF1 is essential for RING-mediated H3 ubiquitylation. Using chemical crosslinking and mass spectrometry, biochemical assays, and recombinant chromatin substrates, we show that the UBL participates in structural rearrangements of UHRF1 upon binding to chromatin and the E2 ubiquitin conjugating enzyme UbcH5a/UBE2D1. Similar to ubiquitin, the UBL exerts its effects through a hydrophobic patch that contacts a regulatory surface on the "backside'' of the E2 to stabilize the E2-E3-chromatin complex. Our analysis of the enzymatic mechanism of UHRF1 uncovers an unexpected function of the UBL domain and defines a new role for this domain in DNMT1-dependent inheritance of DNA methylation.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Chromatin ; Dna Methylation ; Histone Modification ; Ubiquitin-like Fold ; Ubiquitylation ; Uhrf1; Maintenance Dna Methylation; Histone H3 Tail; Epigenetic Inheritance; Crystal-structure; Recognition; Dnmt1; Protein; Activation; Reveals; Association
Language
english
Publication Year
2018
HGF-reported in Year
2018
ISSN (print) / ISBN
1097-2765
e-ISSN
1097-4164
Journal
Molecular Cell
Quellenangaben
Volume: 72,
Issue: 4,
Pages: 739-752
Publisher
Elsevier
Publishing Place
50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Reviewing status
Peer reviewed
Institute(s)
Institute of Functional Epigenetics (IFE)
POF-Topic(s)
30203 - Molecular Targets and Therapies
Research field(s)
Helmholtz Diabetes Center
PSP Element(s)
G-502800-001
WOS ID
WOS:000450187900014
Scopus ID
85056578940
PubMed ID
30392929
Erfassungsdatum
2018-11-07