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Théry, C.* ; Witwer, K.W.* ; Aikawa, E.* ; Alcaraz, M.J.* ; Anderson, J.D.* ; Andriantsitohaina, R.* ; Antoniou, A.C.* ; Arab, T.* ; Archer, F.* ; Atkin-Smith, G.K.* ; Ayre, D.C.* ; Bach, J.M.* ; Bachurski, D.* ; Baharvand, H.* ; Balaj, L.* ; Baldacchino, S.* ; Bauer, N.N.* ; Baxter, A.A.* ; Bebawy, M.* ; Beckham, C.* ; Bedina Zavec, A.* ; Benmoussa, A.* ; Berardi, A.C.* ; Bergese, P.* ; Bielska, E.* ; Blenkiron, C.* ; Bobis-Wozowicz, S.* ; Boilard, E.* ; Boireau, W.* ; Bongiovanni, A.* ; Borràs, F.E.* ; Bosch, S.* ; Boulanger, C.M.* ; Breakefield, X.* ; Breglio, A.M.* ; Brennan, M.* ; Brigstock, D.R.* ; Brisson, A.* ; Broekman, M.L.D.* ; Bromberg, J.F.* ; Bryl-Górecka, P.* ; Buch, S.* ; Buck, A.H.* ; Burger, D.* ; Busatto, S.* ; Buschmann, D.* ; Bussolati, B.* ; Buzás, E.I.* ; Byrd, J.B.* ; Camussi, G.* ; Carter, D.R.F.* ; Gärtner, K. ; Kawamura, Y.* ; Keller, E.T.* ; Khamari, D.* ; Chen, C.* ; Chen, S.* ; Cheng, L.* ; Chin, A.R.* ; Clayton, A.* ; Clerici, S.P.* ; Cocks, A.* ; Cocucci, E.* ; Coffey, R.J.* ; Cordeiro-da-Silva, A.* ; Couch, Y.* ; Coumans, F.A.W.* ; Coyle, B.* ; Crescitelli, R.* ; Criado, M.F.* ; D’Souza-Schorey, C.* ; Das, S.* ; de Candia, P.* ; De Santana, E.F.*

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines.

J. Extra. Vesicles 7, 1535750 (2018)
Publ. Version/Full Text DOI PMC
Open Access Gold
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The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Ectosomes ; Exosomes ; Extracellular Vesicles ; Guidelines ; Microparticles ; Microvesicles ; Minimal Information Requirements ; Reproducibility ; Rigor ; Standardization; Cell-derived Microparticles; Surface-plasmon Resonance; Size-exclusion Chromatography; Field-flow Fractionation; Human Urinary Exosomes; Prostate-cancer; Membrane-vesicles; Fetal Bovine; Circulating Microparticles; Preanalytical Parameters
Language english
Publication Year 2018
HGF-reported in Year 2018
ISSN (print) / ISBN 2001-3078
e-ISSN 2001-3078
Journal Journal of extracellular vesicles
Quellenangaben Volume: 7, Issue: 1, Pages: 1535750 Article Number: , Supplement: ,
Publisher Taylor & Francis
Publishing Place [S.l.]
Reviewing status Peer reviewed
Institute(s) Research Unit Gene Vector (AGV)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Immune Response and Infection
PSP Element(s) G-501500-001
DOI 10.1080/20013078.2018.1535750
WOS ID WOS:000452068900001
WOS ID WOS:000451147500001
Scopus ID 85057143501
PubMed ID 30637094
Erfassungsdatum 2018-12-07
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