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Open Access Green as soon as Postprint is submitted to ZB.
Quantification of aneuploidy in mammalian systems.
Methods Mol. Biol. 1896, 159-190 (2019)
High-throughput next generation sequencing karyotyping has emerged as a powerful tool for the detection of genomic heterogeneity in normal tissues and cancers. Here we describe a single-cell whole genome sequencing (scWGS) platform to assess whole-chromosome aneuploidy, structural aneuploidies involving only chromosome fragments and more local small copy number alterations in individual cells. We provide a detailed protocol for the isolation, library preparation, low coverage sequencing and data analysis of single cells. Since our approach does not involve a whole-genome preamplification step, our method allows for acquisition of reliable high-resolution single-cell copy number profiles. Moreover, the protocol allows multiplexing of 384 single-cell libraries in one sequencing run, thereby significantly reducing sequencing costs and can be completed in 3-4 days starting from single cell isolation to analysis of sequencing data.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Aneuploidy ; Copy Number Alterations ; Library Preparation ; Single-cell Whole Genome Sequencing
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Cellular Senescence
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1896,
Pages: 159-190
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Computational Biology (ICB)