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Capillary zone electrophoresis coupled to drift tube ion mobility-mass spectrometry for the analysis of native and APTS-labeled N-glycans.
Anal. Bioanal. Chem. 411, 6255-6264 (2018)
Capillary zone electrophoresis (CZE) based on electrophoretic mobility in the liquid phase and ion mobility spectrometry (IMS) based on mobilities in the gas phase are both powerful techniques for the separation of complex samples. Protein glycosylation is one of the most common post-translational modifications associated with a wide range of biological functions and human diseases. Due to their high structural variability, the analysis of glycans still represents a challenging task. In this work, the first on-line coupling of CZE with drift tube ion mobility-mass spectrometry (DTIM-MS) has been perfomed to further improve separation capabilities for the analysis of native and 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled N-glycans. In this way, a complexity of glycan signals was revealed which could not be resolved by these techniques individually, shown for both native and APTS-labeled glycans. Each individual glycan signal separated in CZE exhibited an unexpectedly high number of peaks observed in the IMS dimension. This observation could potentially be explained by the presence of isomeric forms, including different linkages, and/or gas-phase conformers. In addition, the type of sialic acid attached to glycans has a significant impact on the obtained drift time profile. Furthermore, the application of alpha 2-3 neuraminidase enabled the partial assignment of peaks in the arrival time distribution considering their sialic acid linkages (alpha 2-3/alpha 2-6). This work is a showcase for the high potential of CZE-DTIM-MS, which is expected to find various applications in the future.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Glycan Analysis ; Ce-im-ms ; Isomer Separation ; Liquid ; And Gas-phase Separation; Oligosaccharides; Identification; Glycosylation; Separation
ISSN (print) / ISBN
1618-2642
e-ISSN
1618-2650
Quellenangaben
Volume: 411,
Issue: 24,
Pages: 6255-6264
Publisher
Springer
Publishing Place
Heidelberg
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Research Unit Analytical BioGeoChemistry (BGC)