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Schulz, S.* ; Pérez-de-Mora, A.* ; Engel, M. ; Munch, J.-C.* ; Schloter, M.

A comparative study of most probable number (MPN)-PCR vs. real-time-PCR for the measurement of abundance and assessment of diversity of alkB homologous genes in soil.

J. Microbiol. Methods 80, 295-298 (2010)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The utilization of quantitative PCR (qPCR) approaches such as MPN-qPCR and real-time-qPCR for in situ assessment of functional genes yields substantial quantitative and qualitative differences. We show this by targeting the alkB gene related to biodegradation of aliphatic alkanes in a set of environmental samples with differing hydrocarbon content.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Quantitative PCR; Real-time PCR; MPN-PCR; Alkane monooxygenase gene alkB
ISSN (print) / ISBN 0167-7012
e-ISSN 1872-8359
Journal Journal of Microbiological Methods
Quellenangaben Volume: 80, Issue: 3, Pages: 295-298 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Amsterdam
Reviewing status Peer reviewed
Institute(s) Institute of Soil Ecology (IBOE)