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Ramani, A.* ; Mariappan, A.* ; Gottardo, M.* ; Mandad, S.* ; Urlaub, H.* ; Avidor-Reiss, T.* ; Riparbelli, M.* ; Callaini, G.* ; Debec, A.* ; Feederle, R. ; Gopalakrishnan, J.*

Plk1/Polo phosphorylates Sas-4 at the onset of mitosis for an efficient recruitment of pericentriolar material to centrosomes.

Cell Rep. 25, 3618-3630.e6 (2018)

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Centrosomes are the major microtubule-organizing centers, consisting of centrioles surrounded by a pericentriolar material (PCM). Centrosomal PCM is spatiotemporally regulated to be minimal during interphase and expands as cells enter mitosis. It is unclear how PCM expansion is initiated at the onset of mitosis. Here, we identify that, in Drosophila, Plk1/Polo kinase phosphorylates the conserved centrosomal protein Sas-4 in vitro. This phosphorylation appears to occur at the onset of mitosis, enabling Sas-4's localization to expand outward from meiotic and mitotic centrosomes. The Plk1/Polo kinase site of Sas-4 is then required for an efficient recruitment of Cnn and gamma-tubulin, bona fide PCM proteins that are essential for PCM expansion and centrosome maturation. Point mutations at Plk1/Polo sites of Sas-4 affect neither centrosome structure nor centriole duplication but specifically reduce the affinity to bind Cnn and gamma-tubulin. These observations identify Plk1/Polo kinase regulation of Sas-4 as essential for efficient PCM expansion.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Drosophila Melanogaster ; Plk1 ; Sas-4 ; Centrosome Maturation ; Centrosomes ; Pericentriolar Material; Daughter Centrioles; Drosophila Spd-2; Gamma-tubulin; Polo; Size; Asterless; Kinase; Cpap; Pcm

ISSN (print) / ISBN 2211-1247

e-ISSN 2211-1247

Journal Cell Reports

Quellenangaben Volume: 25, Issue: 13, Pages: 3618-3630.e6

Publisher Cell Press

Publishing Place 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CF Monoclonal Antibodies (CF-MAB)