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Open Access Gold as soon as Publ. Version/Full Text is submitted to ZB.
Wide-field three-photon excitation in biological samples.
Light Sci. Appl. 6:e16255 (2017)
Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Biophotonics ; Multiphoton Microscopy ; Optogenetics ; Temporal Focusing ; Three-photon
ISSN (print) / ISBN
2047-7538
e-ISSN
2047-7538
Journal
Light: Science & Applications
Quellenangaben
Volume: 6,
Article Number: e16255
Publisher
Nature Publishing Group
Publishing Place
London
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Helmholtz Pioneer Campus (HPC)