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Obermann, J. ; Wagner, F. ; Kociaj, A. ; Zambusi, A. ; Ninkovic, J. ; Hauck, S.M. ; Chapouton, P.

The surface proteome of adult neural stem cells in zebrafish unveils long-range cell-cell connections and age-related changes in responsiveness to IGF.

Stem Cell Rep. 12, 258-273 (2019)

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In adult stem cell populations, recruitment into division is parsimonious and most cells maintain a quiescent state. How individual cells decide to enter the cell cycle and how they coordinate their activity remains an essential problem to be resolved. It is thus important to develop methods to elucidate the mechanisms of cell communication and recruitment into the cell cycle. We made use of the advantageous architecture of the adult zebrafish telencephalon to isolate the surface proteins of an intact neural stem cell (NSC) population. We identified the proteome of NSCs in young and old brains. The data revealed a group of proteins involved in filopodia, which we validated by a morphological analysis of single cells, showing apically located cellular extensions. We further identified an age-related decrease in insulin-like growth factor (IGF) receptors. Expressing IGF2b induced divisions in young brains but resulted in incomplete divisions in old brains, stressing the role of cell-intrinsic processes in stem cell behavior.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Gfap ; Aging ; Biotinylation ; Filopodia ; Lamellipodia ; Mass Spectrometry ; Neurogenesis ; Pallium ; Quiescence ; Radial Glia ; Telencephalon; Radial Glia; Hypothalamic Neurogenesis; Subventricular Zone; Ependymal Cells; Growth; Brain; Quiescence; Decline; Cycle; Proliferation

ISSN (print) / ISBN 2213-6711

Journal Stem Cell Reports

Quellenangaben Volume: 12, Issue: 2, Pages: 258-273

Publisher Cell Press

Publishing Place Maryland Heights, MO

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Sensory Biology and Organogenesis (SBO)
CF Metabolomics & Proteomics (CF-MPC)
Institute of Stem Cell Research (ISF)