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Raabe, N.* ; Forberich, E.* ; Freund, B.* ; Bruns, O.T. ; Heine, M.* ; Kaul, M.G.* ; Tromsdorf, U.* ; Herich, L.* ; Nielsen, P.* ; Reimer, R.* ; Hohenberg, H.* ; Weller, H.* ; Schumacher, U.* ; Adam, G.* ; Ittrich, H.*

Determination of liver-specific r2 * of a highly monodisperse USPIO by 59Fe iron core-labeling in mice at 3 T MRI.

Contrast Media Mol. Imaging 10, 153-62 (2015)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Accurate determination of tissue concentration of ultrasmall superparamagnetic iron oxide nanoparticles (USPIO) using T2 * MR relaxometry is still challenging. We present a reliable quantification method for local USPIO amount with the estimation of the liver specific relaxivity r2 * using monodisperse (59) Fe-core-labeled USPIO ((59) FeUSPIO). Dynamic and relaxometric in vivo characteristics of unlabeled monodisperse USPIO were determined in MRI at 3 T. The in vivo MR studies were performed for liver tissue with (59) FeUSPIO using iron dosages of 9 (n = 3), 18 (n = 2) and 27 (n = 3) µmol Fe kg(-1) body weight. The R2 * of the liver before and after USPIO injection (∆R2 *) was measured and correlated with (59) Fe activity measurements of excised organs by a whole body radioactivity counter (HAMCO) to define the dependency of ∆R2 * and (59) FeUSPIO liver concentration and calculate the r2 * of (59) FeUSPIO for the liver. Ultrastructural analysis of liver uptake was performed by histology and transmission electron microscopy. ∆R2 * of the liver revealed a dosage-dependent accumulation of (59) FeUSPIO with a percentage uptake of 70-88% of the injection dose. Hepatic ∆R2 * showed a dose-dependent linear correlation to (59) FeUSPIO activity measurements (r = 0.92) and an r2 * in the liver of 481 ± 74.9 mm(-1) s(-1) in comparison to an in vitro r2 * of 60.5 ± 3.3 mm(-1) s(-1) . Our results indicate that core-labeled (59) FeUSPIO can be used to quantify the local amount of USPIO and to estimate the liver-specific relaxivity r2 *.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords 3t Mri ; Uspio ; Animal Study ; Quantification ; Relaxometry
ISSN (print) / ISBN 1555-4309
e-ISSN 1555-4317
Journal Contrast Media & Molecular Imaging
Quellenangaben Volume: 10, Issue: 2, Pages: 153-62 Article Number: , Supplement: ,
Publisher Wiley
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)