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Martinez Jimenez, C.P. ; Gómez-Lechón, M.J.* ; von Castell, J.* ; Jover, R.*

Engineering human hepatoma cells with key transcription factors to generate metabolically competent hepatic models.

ALTEX, 435-440 (2005)

Open Access Green as soon as Postprint is submitted to ZB.

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Because of the intrinsic variability and limited accessibility of primary cultured human hepatocytes, other cell models (e.g. cell lines) have been considered in drug metabolism studies. Unfortunately, hepatic cell lines express only marginal levels of drug-metabolising cytochrome P450s (CYPs) and are not a real alternative. Lack of CYP expression in hepatomas appears to be the consequence of an altered expression of liver-enriched transcription factors and co-regulators. We have attempted to up-regulate CYP expression by transfecting hepatoma cells with selected factors. Re-expression of one or several of these factors proved to be effective in re-activating different CYPs, and supports the notion that this can be an appropriate strategy to achieve metabolically competent cell lines.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

ISSN (print) / ISBN 0946-7785

Journal Alternatives to Animal Experimentation : ALTEX

Quellenangaben Pages: 435-440

Publisher Springer

Non-patent literature Publications

Institute(s) Helmholtz Pioneer Campus (HPC)