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Grossmann, G.* ; Guo, W.J.* ; Ehrhardt, D.W.* ; Frommer, W.B.* ; Sit, R.V.* ; Quake, S.R.* ; Meier, M.

The RootChip: An integrated microfluidic chip for plant science.

Plant Cell 23, 4234-4240 (2011)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
Studying development and physiology of growing roots is challenging due to limitations regarding cellular and subcellular analysis under controlled environmental conditions. We describe a microfluidic chip platform, called RootChip, that integrates live-cell imaging of growth and metabolism of Arabidopsis thaliana roots with rapid modulation of environmental conditions. The RootChip has separate chambers for individual regulation of the microenvironment of multiple roots from multiple seedlings in parallel. We demonstrate the utility of The RootChip by monitoring time-resolved growth and cytosolic sugar levels at subcellular resolution in plants by a genetically encoded fluorescence sensor for glucose and galactose. The RootChip can be modified for use with roots from other plant species by adapting the chamber geometry and facilitates the systematic analysis of root growth and metabolism from multiple seedlings, paving the way for large-scale phenotyping of root metabolism and signaling.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
ISSN (print) / ISBN 1040-4651
e-ISSN 1532-298X
Journal The Plant cell
Quellenangaben Volume: 23, Issue: 12, Pages: 4234-4240 Article Number: , Supplement: ,
Publisher American Society of Plant Biologists (ASPB)
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)