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Open Access Green as soon as Postprint is submitted to ZB.
Investigating age-related changes in proliferation and the cell division repertoire of parenchymal reactive astrocytes.
In: Astrocytes. Berlin [u.a.]: Springer, 2019. 277-292 (Methods Mol. Biol. ; 1938)
Reactive gliosis is a complicated process involving all types of glial cells and is the therapeutic target of efforts to treat several types of neuropathologies. Parenchymal astrocytes continuously survey their microenvironment to identify even tiny abnormalities in the central nervous system (CNS) homeostasis and react rapidly to brain damage, such as following ischemia, trauma, or neurodegenerative diseases, to prevent propagation of tissue damage. Aging can play causal roles in certain astroglial dysfunctions, however, still little is known to what extent the heterogeneous reaction of astrocytes at the injury site might be impaired over the course of aging. Based on our experience with both in vitro and in vivo experimental paradigms, we describe here in detail the analysis of age-related changes in (1) proliferative response of parenchymal astrocytes within the posttraumatic cerebral cortex grey matter (GM), and (2) repertoire of their cell divisions in adherent cell culture prepared from the injured GM of young and old double transgenic GFAP-mRFP1/(FUCCI)-S/G2/M-mAG-hGeminin mice by single cell time-lapse imaging.
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Publication type
Article: Edited volume or book chapter
Keywords
Brain Injury ; Cell Cycle ; Cell Division ; Cell Imaging ; Proliferation ; Reactive Astrocyte
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Astrocytes
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1938,
Pages: 277-292
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Stem Cell Research (ISF)