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Open Access Green as soon as Postprint is submitted to ZB.
Assessing calcium-stimulated mitochondrial bioenergetics using the seahorse XF96 analyzer.
In: Calcium Signalling. Berlin [u.a.]: Springer, 2019. 197-222 (Methods Mol. Biol. ; 1925)
The development of fluorescence-based oxygen sensors coupled with microplate-based assays for quantitative bioenergetics analyses enables screening multiple experimental conditions at once with small biological material and in a timely manner. In this chapter, we outline detailed protocols and practical tips to design and perform controlled measurements of (a) respiratory and glycolytic metabolism of intact cells, (b) substrate-dependent respiration in permeabilized cells and isolated mitochondria, and (c) calcium-dependent regulation of mitochondrial bioenergetics with Seahorse XF Flux Analyzers.
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Publication type
Article: Edited volume or book chapter
Keywords
Bioenergetics ; Calcium ; Intact Cells ; Mitochondria ; Oxygen Consumption ; Permeabilized Cells ; Respiration ; Seahorse Xf Analyzer
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Calcium Signalling
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1925,
Pages: 197-222
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Diabetes and Obesity (IDO)