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Open Access Green as soon as Postprint is submitted to ZB.
The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.
J. Cell Biol. 189, 497-510 (2010)
Type V myosin (MyoV)-dependent transport of cargo is an essential process in eukaryotes. Studies on yeast and vertebrate MyoV showed that their globular tails mediate binding to the cargo complexes. In Saccharomyces cerevisiae, the MyoV motor Myo4p interacts with She3p to localize asymmetric synthesis of HO 1 (ASH1) mRNA into the bud of dividing cells. A recent study showed that localization of GFP-MS2-tethered ASH1 particles does not require the Myo4p globular tail, challenging the supposed role of this domain. We assessed ASH1 mRNA and Myo4p distribution more directly and found that their localization is impaired in cells expressing globular tail-lacking Myo4p. In vitro studies further show that the globular tail together with a more N-terminal linker region is required for efficient She3p binding. We also determined the x-ray structure of the Myo4p globular tail and identify a conserved surface patch important for She3p binding. The structure shows pronounced similarities to membrane-tethering complexes and indicates that Myo4p may not undergo auto-inhibition of its motor domain.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Class-V myosin; Budding yeast depends; Saccharomyces-cerevisiae; Endoplasmic-reticulum; Molecular-graphics; Monomeric myosin; Transport; Protein; Domain; Motor
ISSN (print) / ISBN
0021-9525
e-ISSN
1540-8140
Journal
Journal of Cell Biology, The
Quellenangaben
Volume: 189,
Issue: 3,
Pages: 497-510
Publisher
Rockefeller University Press
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Structural Biology (STB)