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Kim, H.* ; Kang, H.* ; Kwon, Y. ; Choi, J.* ; Chang, J.H.*

Proportional subcellular localization of Arabidopsis thaliana RabA1a.

Plant Signal. Behav. 14:e1581561 (2019)
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Subcellular localization of trafficking proteins in a single cell affects the assembly of trafficking machinery between organelles and vesicles throughout the targeting pathway. RabGTPase is one of the regulators to direct specific targeting of cargo molecules depending on GDP/GTP bound status. We have recently determined the crystal structures of GDP-bound inactive and both GTP- and GppNHp-bound active forms of Arabidopsis RabA1a. It is notable that the switch regions of RabA1a exhibit conformational changes derived by GDP or GTP binding. However, it was not clear that where the GDP- or GTP-bound RabA1a is localized at the subcellular level in a cell. Here we demonstrate that the distinct proportion of subcellular localization of RabA1a depends on its site-specific mutation as the GDP- or GTP-bound form. RabA1a proteins located at the plasma membrane, endosomes, and cytosol. While the GDP-bound form of RabA1a(S27N) located more at endosomes than the plasma membrane compared to the proportions of RabA1a wild-type, and the GTP-bound RabA1a(Q72L) located mainly at the plasma membrane in comparison to RabA1a wild-type and RabA1a(S27N). These distinct proportional localizations of RabA1a enable a cognate interaction between inactive/active RabA1 and effector molecules to direct specific targeting of its cargo molecules.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Raba1a ; Raba1a(s27n) ; Raba1a(q72l) ; Rabgtpase ; Subcellular Localization; Trans-golgi Network; Plasma-membrane; Gtpases; Trafficking; Family
ISSN (print) / ISBN 1559-2316
e-ISSN 1559-2324
Journal Plant Signaling & Behavior
Quellenangaben Volume: 14, Issue: 3, Pages: , Article Number: e1581561 Supplement: ,
Publisher Landes Bioscience
Publishing Place 530 Walnut Street, Ste 850, Philadelphia, Pa 19106 Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Cancer (IDC)