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Duin, S.* ; Schütz, K.* ; Ahlfeld, T.* ; Lehmann, S. ; Lode, A.* ; Ludwig, B. ; Gelinsky, M.*

3D bioprinting of functional islets of Langerhans in an alginate/methylcellulose hydrogel blend.

Adv. Healthc. Mater. 8:e1801631 (2019)

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Open Access Green as soon as Postprint is submitted to ZB.

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Transplantation of pancreatic islets is a promising strategy to alleviate the unstable blood-glucose control that some patients with diabetes type 1 exhibit and has seen many advances over the years. Protection of transplanted islets from the immune system can be accomplished by encapsulation within a hydrogel, the most investigated of which is alginate. In this study, islet encapsulation is combined with 3D extrusion bioprinting, an additive manufacturing method which enables the fabrication of 3D structures with a precise geometry to produce macroporous hydrogel constructs with embedded islets. Using a plottable hydrogel blend consisting of clinically approved ultrapure alginate and methylcellulose (Alg/MC) enables encapsulating pancreatic islets in macroporous 3D hydrogel constructs of defined geometry while retaining their viability, morphology, and functionality. Diffusion of glucose and insulin in the Alg/MC hydrogel is comparable to diffusion in plain alginate; the embedded islets continuously produce insulin and glucagon throughout the observation and still react to glucose stimulation albeit to a lesser degree than control islets.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords 3d Bioprinting ; Alginate ; Diabetes ; Insulin ; Macroporous ; Pancreatic Islet; Pancreatic-islets; In-vitro; Rat Islets; Nonimmunosuppressed Patients; Insulin-secretion; Beta-cells; Adult-rat; Alginate; Transplantation; Encapsulation

ISSN (print) / ISBN 2192-2640

e-ISSN 2192-2659

Journal Advanced healthcare materials

Quellenangaben Volume: 8, Issue: 7, Article Number: e1801631

Publisher Wiley

Publishing Place Weinheim

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute for Pancreatic Beta Cell Research (IPI)