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as soon as is submitted to ZB.
Design and generation of gene-targeting vectors.
In: Current Protocols in Mouse Biology. Hoboken, NJ: Wiley-Blackwell, 2011. 199-211 ( ; 1)
This unit provides an overview of the major types of mutant alleles that can be generated by gene targeting in ES cells. It presents the growing public resources of premade gene targeting vectors, modified ES cells, and mutant mice. General guidelines for the design of targeting vectors are followed by protocols for the construction of vectors to generate knockout (KO), conditional KO, and subtle mutant alleles.
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Publication type
Article: Periodical or book chapter
Keywords
gene targeting; conditional KO mice; targeting vector design; knockout mice; EUCOMM/KOMP
ISBN
9780470942390
Book Volume Title
Current Protocols in Mouse Biology
Quellenangaben
Volume: 1,
Pages: 199-211
Publisher
Wiley-Blackwell
Publishing Place
Hoboken, NJ
Institute(s)
Institute of Developmental Genetics (IDG)