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Meyer, M. ; Hrabě de Angelis, M. ; Wurst, W. ; Kühn, R.

Gene targeting by homologous recombination in mouse zygotes mediated by zinc-finger nucleases.

Proc. Natl. Acad. Sci. U.S.A. 107, 15022-15026 (2010)
DOI PMC
Gene targeting by homologous recombination in embryonic stem cells is extensively used to generate specific mouse mutants. However, most mammalian species lack tools for targeted gene manipulation. Since double-strand breaks strongly increase the rate of homologous recombination at genomic loci, we explored whether gene targeting can be directly performed in zygotes by the use of zinc-finger nucleases. Here we report that gene targeting is achieved in 1.7-4.5% of murine one-cell embryos upon the coinjection of targeting vectors with zinc-finger nucleases, without preselection. These findings enable the manipulation of the mammalian germ line in a single step in zygotes, independent of ES cells.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Mammalian-cells; Knockout rats; Mice
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Journal Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Volume: 107, Issue: 34, Pages: 15022-15026 Article Number: , Supplement: ,
Publisher National Academy of Sciences
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Developmental Genetics (IDG)
Institute of Experimental Genetics (IEG)