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Gottschalk, S. ; Degtyaruk, O. ; Mc Larney, B. ; Rebling, J. ; Dean-Ben, X.L. ; Shoham, S.* ; Razansky, D.

Isolated murine brain model for large-scale optoacoustic calcium imaging.

Front. Neurosci. 13:290 (2019)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
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Real-time visualization of large-scale neural dynamics in whole mammalian brains is hindered with existing neuroimaging methods having limited capacity when it comes to imaging large tissue volumes at high speeds. Optoacoustic imaging has been shown to be capable of real-time three-dimensional imaging of multiple cerebral hemodynamic parameters in rodents. However, optoacoustic imaging of calcium activity deep within the mammalian brain is hampered by strong blood absorption in the visible light spectrum as well as a lack of activity labels excitable in the near-infrared window. We have developed and validated an isolated whole mouse brain preparation labeled with genetically encoded calcium indicator GCaMP6f, which can closely resemble in vivo conditions. An optoacoustic imaging system coupled to a superfusion system was further designed and used for rapid volumetric monitoring of stimulus-evoked calcium dynamics in the brain. These new imaging setup and isolated preparation's protocols and characteristics are described here in detail. Our new technique captures calcium fluxes as true three-dimensional information across the entire brain with temporal resolution of 10 ms and spatial resolution of 150 μm, thus enabling large-scale neural recording at penetration depths and spatio-temporal resolution scales not covered with any existing neuroimaging techniques.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Gcamp6f ; Calcium Dynamics ; Functional Neuroimaging ; Isolated Brain ; Optoacoustic Neuroimaging; Vivo; Tomography; Ketamine; Culture; Cells
ISSN (print) / ISBN 1662-453X
Journal Frontiers in Neuroscience
Quellenangaben Volume: 13, Issue: , Pages: , Article Number: 290 Supplement: ,
Publisher Frontiers
Publishing Place Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland
Non-patent literature Publications
Institute(s) Institute of Biological and Medical Imaging (IBMI)