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Dombrowsky, A. ; Schauer, J.* ; Sammer, M.* ; Blutke, A. ; Walsh, D.W.M.* ; Schwarz, B.* ; Bartzsch, S. ; Feuchtinger, A. ; Reindl, J.* ; Combs, S.E. ; Dollinger, G.* ; Schmid, T.E.

Acute skin damage and late radiation-induced fibrosis and inflammation in murine ears after high-dose irradiation.

Cancers 11:727 (2019)
Publ. Version/Full Text DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The use of different scoring systems for radiation-induced toxicity limits comparability between studies. We examined dose-dependent tissue alterations following hypofractionated X-ray irradiation and evaluated their use as scoring criteria. Four dose fractions (0, 5, 10, 20, 30 Gy/fraction) were applied daily to ear pinnae. Acute effects (ear thickness, erythema, desquamation) were monitored for 92 days after fraction 1. Late effects (chronic inflammation, fibrosis) and the presence of transforming growth factor beta 1 (TGF beta 1)-expressing cells were quantified on day 92. The maximum ear thickness displayed a significant positive correlation with fractional dose. Increased ear thickness and erythema occurred simultaneously, followed by desquamation from day 10 onwards. A significant dose-dependency was observed for the severity of erythema, but not for desquamation. After 4 x 20 and 4 x 30 Gy, inflammation was significantly increased on day 92, whereas fibrosis and the abundance of TGF beta 1-expressing cells were only marginally increased after 4 x 30 Gy. Ear thickness significantly correlated with the severity of inflammation and fibrosis on day 92, but not with the number of TGF beta 1-expressing cells. Fibrosis correlated significantly with inflammation and fractional dose. In conclusion, the parameter of ear thickness can be used as an objective, numerical and dose-dependent quantification criterion to characterize the severity of acute toxicity and allow for the prediction of late effects.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Hypofractionation ; Side Effects ; Acute ; Late ; High-dose ; Fractionated Radiotherapy ; Skin; Mouse Skin; Ionizing-radiation; Therapy; Expression; Dermatitis; Mechanisms; Epidermis; Toxicity; Cancer
ISSN (print) / ISBN 2072-6694
Journal Cancers
Quellenangaben Volume: 11, Issue: 5, Pages: , Article Number: 727 Supplement: ,
Publisher MDPI
Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Medicine (IRM)
Research Unit Analytical Pathology (AAP)