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Vieira Braga, F.A.* ; Kar, G.* ; Berg, M.* ; Carpaij, O.A.* ; Polanski, K.* ; Simon, L. ; Brouwer, S.* ; Gomes, T.* ; Hesse, L.* ; Jiang, J.* ; Fasouli, E.S.* ; Efremova, M.* ; Vento-Tormo, R.* ; Talavera-López, C.* ; Jonker, M.R.* ; Affleck, K.* ; Palit, S. ; Strzelecka, P.M.* ; Firth, H.V.* ; Mahbubani, K.T.* ; Cvejic, A.* ; Meyer, K.B.* ; Saeb-Parsy, K.* ; Luinge, M.* ; Brandsma, C.A.* ; Timens, W.* ; Angelidis, I. ; Strunz, M. ; Koppelman, G.H.* ; van Oosterhout, A.J.* ; Schiller, H. B. ; Theis, F.J. ; van den Berge, M.* ; Nawijn, M.C.* ; Teichmann, S.A.*

A cellular census of human lungs identifies novel cell states in health and in asthma.

Nat. Med. 25, 1153-1163 (2019)
Postprint Research data DOI PMC
Open Access Green
Human lungs enable efficient gas exchange and form an interface with the environment, which depends on mucosal immunity for protection against infectious agents. Tightly controlled interactions between structural and immune cells are required to maintain lung homeostasis. Here, we use single-cell transcriptomics to chart the cellular landscape of upper and lower airways and lung parenchyma in healthy lungs, and lower airways in asthmatic lungs. We report location-dependent airway epithelial cell states and a novel subset of tissue-resident memory T cells. In the lower airways of patients with asthma, mucous cell hyperplasia is shown to stem from a novel mucous ciliated cell state, as well as goblet cell hyperplasia. We report the presence of pathogenic effector type 2 helper T cells (T(H)2) in asthmatic lungs and find evidence for type 2 cytokines in maintaining the altered epithelial cell states. Unbiased analysis of cell-cell interactions identifies a shift from airway structural cell communication in healthy lungs to a T(H)2-dominated interactome in asthmatic lungs.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Epithelial-cells; Prostaglandin D-2; Rna-seq; Expression; Inflammation; Pathogenesis; Macrophages; Phenotype; Disease; Gene
Language english
Publication Year 2019
HGF-reported in Year 2019
ISSN (print) / ISBN 1078-8956
e-ISSN 1546-170X
Journal Nature medicine
Quellenangaben Volume: 25, Issue: 7, Pages: 1153-1163 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Reviewing status Peer reviewed
Institute(s) Institute of Computational Biology (ICB)
German Center for Lung Research (DZL)
POF-Topic(s) 30205 - Bioengineering and Digital Health
80000 - German Center for Lung Research
Research field(s) Enabling and Novel Technologies
Lung Research
PSP Element(s) G-503800-001
G-501800-810
DOI 10.1038/s41591-019-0468-5
WOS ID WOS:000474430400030
Scopus ID 85067892275
PubMed ID 31209336
Erfassungsdatum 2019-06-26
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