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Huertas, J.R.* ; Ruiz Ojeda, F.J. ; Plaza-Díaz, J.* ; Nordsborg, N.B.* ; Martín-Albo, J.* ; Rueda-Robles, A.* ; Casuso, R.A.*

Human muscular mitochondrial fusion in athletes during exercise.

FASEB J. 33, 12087-12098 (2019)
DOI PMC
Open Access Green as soon as Postprint is submitted to ZB.
The main objective of this work was to investigate whether mitochondrial fusion occurs in the skeletal muscle of well-trained athletes in response to high-intensity exercise. Well-trained swimmers (n = 9) performed a duration-matched sprint interval training (SIT) and high-intensity high-volume training (HIHVT) session on separate days. Muscle samples from triceps brachii were taken before, immediately after, and 3 h after the training sessions. Transmission electron microscopy (TEM) was applied to assess mitochondrial morphology. Moreover, expression of genes coding for regulators of mitochondrial fusion and fission were assessed by real-time quantitative PCR. In addition, mitofusin (MFN)2 and optic atrophy 1 (OPA1) were quantified by Western blot analysis. TEM analyses showed that mitochondrial morphology remained altered for 3 h after HIHVT, whereas SIT-induced changes were only evident immediately after exercise. Only SIT increased MFN1 and MFN2 mRNA expression, whereas SIT and HIHVT both increased MFN2 protein content 3 h after exercise. Notably, only HIHVT increased OPA1 protein content. Mitochondrial morphologic changes that suggest fusion occurs in well-adapted athletes during exercise. However, HIHVT appears as a more robust inducer of mitochondrial fusion events than SIT. Indeed, SIT induces a rapid and transient change in mitochondrial morphology.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Physical Activity ; Mitochondrial Fission ; Skeletal Muscle ; Mitofusin 2; Human Skeletal-muscle; Membrane Interactions; Fission; Protein; Reticulum; Adaptations; Activation; Fis1
ISSN (print) / ISBN 0892-6638
e-ISSN 1530-6860
Journal FASEB Journal
Quellenangaben Volume: 33, Issue: 11, Pages: 12087-12098 Article Number: , Supplement: ,
Publisher Wiley
Publishing Place Bethesda, Md.
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Diabetes and Obesity (IDO)