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Stanisavljevic, D.* ; Popovic, J.* ; Petrovic, I.* ; Davidovic, S.* ; Atkinson, M.J. ; Anastasov, N. ; Stevanovic, M.*

Radiation effects on early phase of NT2/D1 neural differentiation in vitro.

Int. J. Radiat. Biol. 95, 1627-1639 (2019)
Postprint DOI PMC
Open Access Green
Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of γ radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Markers Of Pluripotency ; Mirnas ; Neural Differentiation ; Nt2/d1 Cell Line ; Radiation
ISSN (print) / ISBN 0955-3002
e-ISSN 1362-3095
Journal International Journal of Radiation Biology
Quellenangaben Volume: 95, Issue: 12, Pages: 1627-1639 Article Number: , Supplement: ,
Publisher Informa Healthcare
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)