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Genome analysis provides insights into microaerobic toluene-degradation pathway of Zoogloea oleivorans Buc(T).
Arch. Microbiol. 202, 421–426 (2019)
Zoogloea oleivorans, capable of using toluene as a sole source of carbon and energy, was earlier found to be an active degrader under microaerobic conditions in aquifer samples. To uncover the genetic background of the ability of microaerobic toluene degradation in Z. oleivorans, the whole-genome sequence of the type strain Buc(T) was revealed. Metatranscriptomic sequence reads, originated from a previous SIP study on microaerobic toluene degradation, were mapped on the genome. The genome (5.68 Mb) had a mean G + C content of 62.5%, 5005 protein coding gene sequences and 80 RNA genes. Annotation predicted that 66 genes were involved in the metabolism of aromatic compounds. Genome analysis revealed the presence of a cluster with genes coding for a multicomponent phenol-hydroxylase system and a complete catechol meta-cleavage pathway. Another cluster flanked by mobile-element protein coding genes coded a partial catechol meta-cleavage pathway including a subfamily I.2.C-type extradiol dioxygenase. Analysis of metatranscriptomic data of a microaerobic toluene-degrading enrichment, containing Z . oleivorans as an active-toluene degrader revealed that a toluene dioxygenase-like enzyme was responsible for the ring-hydroxylation, while enzymes of the partial catechol meta-cleavage pathway coding cluster were responsible for further degradation of the aromatic ring under microaerobic conditions. This further advances our understanding of aromatic hydrocarbon degradation between fully oxic and strictly anoxic conditions.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Zoogloea ; Toluene Degradation ; Metatranscriptomics ; Biodegradation; Sp Nov.; Bacterium
ISSN (print) / ISBN
0003-9276
e-ISSN
1432-072X
Journal
Archives of Microbiology
Quellenangaben
Volume: 202,
Issue: 2,
Pages: 421–426
Publisher
Springer
Publishing Place
233 Spring St, New York, Ny 10013 Usa
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Groundwater Ecology (IGOE)