PuSH - Publication Server of Helmholtz Zentrum München: Noninvasive phenotyping of plant-pathogen interaction: Consecutive in situ imaging of fluorescing Pseudomonas syringae, plant phenolic fluorescence, and chlorophyll fluorescence in Arabidopsis leaves.

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Hupp, S.* ; Rosenkranz, M. ; Bonfig, K.* ; Pandey, C.* ; Roitsch, T.*

Noninvasive phenotyping of plant-pathogen interaction: Consecutive in situ imaging of fluorescing Pseudomonas syringae, plant phenolic fluorescence, and chlorophyll fluorescence in Arabidopsis leaves.

Front. Plant Sci. 10:1239 (2019)

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Plant-pathogen interactions have been widely studied, but mostly from the site of the plant secondary defense. Less is known about the effects of pathogen infection on plant primary metabolism. The possibility to transform a fluorescing protein into prokaryotes is a promising phenotyping tool to follow a bacterial infection in plants in a noninvasive manner. In the present study, virulent and avirulent Pseudomonas syringae strains were transformed with green fluorescent protein (GFP) to follow the spread of bacteria in vivo by imaging Pulse-Amplitude-Modulation (PAM) fluorescence and conventional binocular microscopy. The combination of various wavelengths and filters allowed simultaneous detection of GFP-transformed bacteria, PAM chlorophyll fluorescence, and phenolic fluorescence from pathogen-infected plant leaves. The results show that fluorescence imaging allows spatiotemporal monitoring of pathogen spread as well as phenolic and chlorophyll fluorescence in situ, thus providing a novel means to study complex plant-pathogen interactions and relate the responses of primary and secondary metabolism to pathogen spread and multiplication. The study establishes a deeper understanding of imaging data and their implementation into disease screening.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Green Fluorescence Protein (gfp) ; Plant-pathogen Interaction ; Imaging Pam ; Chlorophyll Fluorescence Imaging ; Phenolic Compounds; Metabolism; Infection; Protein; Photosynthesis; Secondary; Defense; Model

ISSN (print) / ISBN 1664-462X

e-ISSN 1664-462X

Journal Frontiers in Plant Science

Quellenangaben Volume: 10, Article Number: 1239

Publisher Frontiers

Publishing Place Avenue Du Tribunal Federal 34, Lausanne, Ch-1015, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Research Unit Environmental Simulation (EUS)