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Gotthardt, M.* ; Kanawati, B. ; Schmidt, F.* ; Asam, S.* ; Hammerl, R.* ; Frank, O.* ; Hofmann, T.* ; Schmitt-Kopplin, P. ; Rychlik, M.*

Comprehensive analysis of the Alternaria mycobolome using mass spectrometry based metabolomics.

Mol. Nutr. Food Res. 64:1900558 (2020)
Postprint DOI PMC
Open Access Green
Scope Alternaria fungi are widely distributed plant pathogens infecting grains and vegetables and causing major harvest losses in the field and during postharvest storage. Besides, consumers are endangered by the formation of toxic secondary metabolites. Some of these secondary metabolites are chemically characterized as mycotoxins, but the majority of the Alternaria mycobolome still remains unknown. Methods and results Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) and LC-MS/MS are combined for the non-targeted and targeted analysis of the metabolome of three A. alternata isolates and one A. solani isolate. Due to the ultra-high resolution of FTICR-MS, unique molecular formulae are assigned to measured m/z signals. The molecular formulae are matched to entries of the databases Antibase and Kyoto Encyclopedia of Genes and Genomes. The non-targeted analysis of the fungal extracts reveals variations in the secondary metabolite profile of A. alternata and A. solani. Differences in the biosynthesis of dibenzo-alpha-pyrones, perylene quinones, tentoxin, and tenuazonic acid of the A. alternata and A. solani isolates are determined applying targeted LC-MS/MS. Conclusion FTICR-MS analyses reveal clear differences in the metabolic profile of the A. solani and the A. alternata isolates.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Alternaria Alternata ; Alterperylenepoxide A-9-mercaptolactate ; Fourier Transform Ion Cyclotron Resonance Mass Spectrometry ; Non-targeted Metabolomics ; Ultra-high Resolution; (8r,9s)-9,10-epoxy-8-hydroxy-9-methyl-deca-(2e,4z,6e)-trienoic Acid; Masked Mycotoxins; Altertoxin-ii; Methyl-ether; Metabolites; Toxins; Biosynthesis; Fungus; Dihydrotentoxin; Classification
ISSN (print) / ISBN 1613-4125
e-ISSN 1613-4133
Journal Molecular Nutrition and Food Research
Quellenangaben Volume: 64, Issue: 3, Pages: , Article Number: 1900558 Supplement: ,
Publisher Wiley
Publishing Place 111 River St, Hoboken 07030-5774, Nj Usa
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Research Unit Analytical BioGeoChemistry (BGC)