PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
D'Arienzo, V.* ; Magri, A.* ; Harris, J.M.* ; Wing, P.A.C.* ; Ko, C. ; Rubio, C.O.* ; Revill, P.A.* ; Protzer, U. ; Balfe, P.* ; McKeating, J.A.*

A PCR assay to quantify patterns of HBV transcription.

J. Gen. Virol. 102:001373 (2019)
Publ. Version/Full Text DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Hepatitis B virus (HBV) is the prototype member of the family Hepadnaviridae and replicates via episomal copies of a covalently closed circular DNA (cccDNA) genome of approximately 3.2 kb. The chromatinization of this small viral genome, with overlapping open reading frames and regulatory elements, suggests an important role for epigenetic pathways to regulate HBV transcription. However, the host pathways that regulate HBV transcription and the temporal nature of promoter usage in infected cells are not well understood, in part due to the compact genome structure and overlapping open reading frames. To address this we developed a simple and cost-effective PCR assay to quantify the major viral RNAs and validated this technique using current state-of-art de novo HBV infection model systems. Our PCR method is three orders of magnitude more sensitive than Northern blot and requires relatively small amounts of starting material, making this an attractive tool for assessing HBV transcription.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
2.809
0.962
5
5
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Hepatitis B Virus ; Rna ; Transcription
Language english
Publication Year 2019
HGF-reported in Year 2019
ISSN (print) / ISBN 0022-1317
e-ISSN 1465-2099
Journal Journal of General Virology
Quellenangaben Volume: 102, Issue: 3, Pages: , Article Number: 001373 Supplement: ,
Publisher Society for General Microbiology
Publishing Place Charles Darwin House, 12 Roger St, London Wc1n 2ju, Erks, England
Reviewing status Peer reviewed
Institute(s) Institute of Virology (VIRO)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Immune Response and Infection
PSP Element(s) G-502700-003
Grants Wellcome Trust
MRC
EU 2020 Research and Innovation Programme Consortia HEP-CAR
DOI 10.1099/jgv.0.001373
WOS ID WOS:000635958900002
PubMed ID 31846416
Erfassungsdatum 2020-01-15
[➜Report correction]