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Pleitez, M.A. ; Ali Khan, A. ; Solda, A. ; Chmyrov, A. ; Reber, J. ; Gasparin, F. ; Seeger, M. ; Schätz, B. ; Herzig, S. ; Scheideler, M. ; Ntziachristos, V.

Label-free metabolic imaging by mid-infrared optoacoustic microscopy in living cells.

Nat. Biotechnol. 38, 293-296 (2020)
Postprint DOI PMC
Open Access Green
We develop mid-infrared optoacoustic microscopy (MiROM) for label-free, bond-selective, live-cell metabolic imaging, enabling spatiotemporal monitoring of carbohydrates, lipids and proteins in cells and tissues. Using acoustic detection of optical absorption, MiROM converts mid-infrared sensing into a positive-contrast imaging modality with negligible photodamage and high sensitivity. We use MiROM to observe changes in intrinsic carbohydrate distribution from a diffusive spatial pattern to tight co-localization with lipid droplets during adipogenesis.Mid-infrared optoacoustic microscopy enables label-free, bond-selective imaging in living cells
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Raman; Ir
ISSN (print) / ISBN 1087-0156
e-ISSN 1546-1696
Journal Nature Biotechnology
Quellenangaben Volume: 38, Issue: 3, Pages: 293-296 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place New York, NY
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
Institute of Diabetes and Cancer (IDC)