A method of a three-dimensional surface reconstruction of the retina in the area of the papilla is presented. The surface reconstruction is based on a sequence of discrete gray-level images of the retina recorded by a Scanning Laser Ophthalmoscope (SLO). The underlying assumption of the developed surface reconstruction algorithm is that the depth information is also encoded in the brightness values of the single pixels beside the ordinary spatial 2D information. The brightness of an image position depends also on the degree of reflection of a confocal laser beam. Only these surface structures produce a high response of the focused laser light, which are located directly in the focus plane of the confocal laser beam. The occurring disparities between the single images of a sequence are considered to be approximately linear and are corrected by applying the cepstrum technique. The depth information is estimated out of the volumetric representation of the image sequence by searching the maximal value of the brightness within a computed depth profile at every image position. In the resulting range images disturbances which occur during the recording cause wrong local estimations of the depth information. These local disturbances are corrected by applying especially developed surface improvement processes. The work is completed by investigating several different approaches to reduce the noisy and disturbances of SLO image data.