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Chen, Z.* ; Mc Larney, B. ; Rebling, J.* ; Dean-Ben, X.L.* ; Zhou, Q.* ; Gottschalk, S. ; Razansky, D.

High-speed large-field Multifocal illumination fluorescence microscopy.

Laser Photon. Rev. 14:1900070 (2020)
Postprint Research data DOI
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
Scanning optical microscopy techniques are commonly restricted to a sub-millimeter field-of-view (FOV) or otherwise employ slow mechanical translation, limiting their applicability for imaging fast biological dynamics occurring over large areas. A rapid scanning large-field multifocal illumination (LMI) fluorescence microscopy technique is devised based on a beam-splitting grating and an acousto-optic deflector synchronized with a high-speed camera to attain real-time fluorescence microscopy over a centimeter-scale FOV. Owing to its large depth of focus, the approach allows noninvasive visualization of perfusion across the entire mouse cerebral cortex, not achievable with conventional wide-field fluorescence microscopy methods. The new concept can readily be incorporated into conventional wide-field microscopes to mitigate image blur due to tissue scattering and attain optimal trade-off between spatial resolution and FOV. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus it is anticipated to find broad applicability in functional neuroimaging, in vivo cell tracking, and other applications looking at large-scale fluorescent-based biodynamics.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Diffraction Gratings ; Fast Scanning Microscopy ; Fluorescence Imaging ; Multifocal Illumination; 2-photon Microscopy; Neuronal-activity; High-resolution; Excitation; Single; Improves; System
ISSN (print) / ISBN 1863-8880
e-ISSN 1863-8899
Journal Laser & Photonics Reviews
Quellenangaben Volume: 14, Issue: 2, Pages: , Article Number: 1900070 Supplement: ,
Publisher Wiley
Publishing Place Weinheim
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)