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High-speed large-field Multifocal illumination fluorescence microscopy.
Laser Photon. Rev. 14:1900070 (2020)
Scanning optical microscopy techniques are commonly restricted to a sub-millimeter field-of-view (FOV) or otherwise employ slow mechanical translation, limiting their applicability for imaging fast biological dynamics occurring over large areas. A rapid scanning large-field multifocal illumination (LMI) fluorescence microscopy technique is devised based on a beam-splitting grating and an acousto-optic deflector synchronized with a high-speed camera to attain real-time fluorescence microscopy over a centimeter-scale FOV. Owing to its large depth of focus, the approach allows noninvasive visualization of perfusion across the entire mouse cerebral cortex, not achievable with conventional wide-field fluorescence microscopy methods. The new concept can readily be incorporated into conventional wide-field microscopes to mitigate image blur due to tissue scattering and attain optimal trade-off between spatial resolution and FOV. It further establishes a bridge between conventional wide-field macroscopy and laser scanning confocal microscopy, thus it is anticipated to find broad applicability in functional neuroimaging, in vivo cell tracking, and other applications looking at large-scale fluorescent-based biodynamics.
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10.655
2.903
10
17
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Diffraction Gratings ; Fast Scanning Microscopy ; Fluorescence Imaging ; Multifocal Illumination; 2-photon Microscopy; Neuronal-activity; High-resolution; Excitation; Single; Improves; System
Language
english
Publication Year
2020
Prepublished in Year
2019
HGF-reported in Year
2019
ISSN (print) / ISBN
1863-8880
e-ISSN
1863-8899
Journal
Laser & Photonics Reviews
Quellenangaben
Volume: 14,
Issue: 2,
Article Number: 1900070
Publisher
Wiley
Publishing Place
Weinheim
Reviewing status
Peer reviewed
Institute(s)
Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s)
30205 - Bioengineering and Digital Health
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-505590-001
WOS ID
WOS:000503856000001
Scopus ID
85076891329
Erfassungsdatum
2020-01-10