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Schieferdecker, A.* ; Hörber, S. ; Ums, M.* ; Besemer, B.* ; Bokemeyer, C.* ; Peter, A. ; Weisel, K.*

Comparison of three different serum-free light-chain assays-implications on diagnostic and therapeutic monitoring of multiple myeloma.

Blood Cancer J. 10:2 (2020)

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The measurement of serum-free light chains (FLC) is standard of care in the diagnosis and management of multiple myeloma (MM). The revised international myeloma working group (IMWG) implemented the involved FLC/noninvolved FLC (iFLC/niFLC) ratio as a biomarker for MM requiring treatment. Recently, a new definition of high-risk smoldering MM (SMM) including iFLC/niFLC ratio was published. These recommendations were solely based on a single assay method (Freelite assay). Today, two additional assays, N Latex FLC and ELISA-based Sebia FLC, are available. Here, we report on a single-center-study comparing results of all three different assays for FLC correlation and its potential implications for diagnostic and clinical use. In total, 187 samples from 47 MM patients were examined, and determination of FLC was performed. Comparison analyses showed similar FLC results for Sebia FLC and N Latex FLC assay with markedly lower absolute values for kappa/lambda ratio compared with Freelite. Values of lambda FLC exhibited high variability. The ratio of iFLC/niFLC showed significant discrepancies among these assays. Our data demonstrate that the three available assays may result in markedly discrepant results, and should not be used interchangeably to monitor patients. Furthermore, modifications of the assay-specific diagnostic (iFLC/niFLC) thresholds for SMM and MM are recommended.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords N Latex Flc; Criteria; Agreement; Analyzer

e-ISSN 2044-5385

Journal Blood Cancer Journal

Quellenangaben Volume: 10, Issue: 1, Article Number: 2

Publisher Nature Publishing Group

Publishing Place Macmillan Building, 4 Crinan St, London N1 9xw, England

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Diabetes Research and Metabolic Diseases (IDM)