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Abanda-Nkpwatt, D.* ; Müsch, M. ; Tschiersch, J. ; Boettner, M.* ; Schwab, W.*

Molecular interaction between Methylobacterium extorquens and seedling: Growth promotion, methanol consumption and localization of the methanol emission site.

J. Exp. Bot. 57, 4025-4032 (2006)
Publ. Version/Full Text Volltext DOI PMC
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Four Methylobacterium extorquens strains were isolated from strawberry (Fragariaxananassa cv. Elsanta) leaves, and one strain, called ME4, was tested for its ability to promote the growth of various plant seedlings. Seedling weight and shoot length of Nicotiana tabacum, Lycopersicon esculentum, Sinapis alba, and Fragaria vesca increased significantly in the presence of the pink-pigmented facultative methylotroph (PPFM), but the germination behaviour of seeds from six other plants was not affected. The cell-free supernatant of the bacterial culture stimulated germination, suggesting the production of a growth-promoting agent by the methylotroph. Methanol emitted from N. tabacum seedlings, as determined by proton-transfer-reaction mass spectrometry (PTR-MS), ranged from 0.4 to 0.7 ppbv (parts per billion by volume), while significantly lower levels (0.005 to 0.01 ppbv) of the volatile alcohol were measured when the seedlings were co-cultivated with M. extorquens ME4, demonstrating the consumption of the gaseous methanol by the bacteria. Additionally, by using cells of the methylotrophic yeast Pichia pastoris transformed with the pPICHS/GFP vector harbouring a methanol-sensitive promoter in combination with the green fluorescence protein (GFP) reporter gene, stomata were identified as the main source of the methanol emission on tobacco cotyledons. Methylobacterium extorquens strains can nourish themselves using the methanol released by the stomata and release an agent promoting the growth of the seedlings of some crop plants.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Methanol; Methylobacterium extorquens; Nicotiana tabacum; Pichia pastoris; TR-MS; stomata; METHYLOTROPHIC BACTERIA; AEROBIC METHYLOBACTERIA; LEAF SURFACES; IN-VITRO; PLANTS; MORPHOGENESIS; PHYLLOPLANE
ISSN (print) / ISBN 0022-0957
e-ISSN 1460-2431
Journal Journal of Experimental Botany
Quellenangaben Volume: 57, Issue: 15, Pages: 4025-4032 Article Number: , Supplement: ,
Publisher Oxford University Press
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Protection (ISS)