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Open Access Green as soon as Postprint is submitted to ZB.
Bioprinting for human respiratory and gastrointestinal in vitro models.
In: 3D Bioprinting. Berlin [u.a.]: Springer, 2020. 199-215 (Methods Mol. Biol. ; 2140)
Increasing ethical and biological concerns require a paradigm shift toward animal-free testing strategies for drug testing and hazard assessments. To this end, the application of bioprinting technology in the field of biomedicine is driving a rapid progress in tissue engineering. In particular, standardized and reproducible in vitro models produced by three-dimensional (3D) bioprinting technique represent a possible alternative to animal models, enabling in vitro studies relevant to in vivo conditions. The innovative approach of 3D bioprinting allows a spatially controlled deposition of cells and biomaterial in a layer-by-layer fashion providing a platform for engineering reproducible models. However, despite the promising and revolutionizing character of 3D bioprinting technology, standardized protocols providing detailed instructions are lacking. Here, we provide a protocol for the automatized printing of simple alveolar, bronchial, and intestine epithelial cell layers as the basis for more complex respiratory and gastrointestinal tissue models. Such systems will be useful for high-throughput toxicity screening and drug efficacy evaluation.
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Publication type
Article: Edited volume or book chapter
Keywords
Alveolar Epithelial cells ; Bioprinting Technique ; Bronchial Epithelial cells ; In Vitro Cultures ; Intestine Epithelial cells
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
3D Bioprinting
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 2140,
Pages: 199-215
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed