PuSH - Publication Server of Helmholtz Zentrum München: Dry generation of CeO<sub>2</sub> nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

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Cappellini, F.* ; Di Bucchianico, S. ; Karri, V.* ; Latvala, S.* ; Malmlöf, M.* ; Kippler, M.* ; Elihn, K.* ; Hedberg, J.* ; Wallinder, I.O.* ; Gerde, P.* ; Karlsson, H.L.*

Dry generation of CeO₂ nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

Nanomaterials 10:618 (2020)

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Relevant in vitro assays that can simulate exposure to nanoparticles (NPs) via inhalation are urgently needed. Presently, the most common method employed is to expose lung cells under submerged conditions, but the cellular responses to NPs under such conditions might differ from those observed at the more physiological air-liquid interface (ALI). The aim of this study was to investigate the cytotoxic and inflammatory potential of CeO2 NPs (NM-212) in a co-culture of A549 lung epithelial cells and differentiated THP-1 cells in both ALI and submerged conditions. Cellular dose was examined quantitatively using inductively coupled plasma mass spectrometry (ICP-MS). The role of serum and LPS-priming for IL-1 beta release was further tested in THP-1 cells in submerged exposure. An aerosol of CeO2 NPs was generated by using the PreciseInhale (R) system, and NPs were deposited on the co-culture using XposeALI (R). No or minor cytotoxicity and no increased release of inflammatory cytokines (IL-1 beta, IL-6, TNF alpha, MCP-1) were observed after exposure of the co-culture in ALI (max 5 mu g/cm(2)) or submerged (max 22 mu g/cm(2)) conditions. In contrast, CeO2 NPs cause clear IL-1 beta release in monocultures of macrophage-like THP-1, independent of the presence of serum and LPS-priming. This study demonstrates a useful approach for comparing effects at various in-vitro conditions.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Nanotoxicology ; Air-liquid Interface ; Preciseinhale ; Dosimetry ; Inflammation ; Ceria; In-vitro; Toxicity; Aerosols; Size

ISSN (print) / ISBN 2079-4991

e-ISSN 2079-4991

Journal Nanomaterials

Quellenangaben Volume: 10, Issue: 4, Article Number: 618

Publisher MDPI

Publishing Place Basel

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Cooperation Group Comprehensive Molecular Analytics (CMA)

PuSH - Publication Server of Helmholtz Zentrum München

Dry generation of CeO2 nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.

Dry generation of CeO₂ nanoparticles and deposition onto a co-culture of A549 and THP-1 cells in air-liquid interface – dosimetry consideration and comparison to submerged exposure.