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ADAM17 stabilizes its interacting partner inactive Rhomboid 2 (iRhom2) but not inactive Rhomboid 1 (iRhom1).
J. Biol. Chem. 295, 4350-4358 (2020)
The metalloprotease ADAM17 (a disintegrin and metalloprotease 17) is a key regulator of tumor necrosis factor ? (TNF?), interleukin 6 receptor (IL-6R), and epidermal growth factor receptor (EGFR) signaling. ADAM17 maturation and function depend on the seven-membrane?spanning inactive rhomboid-like proteins 1 and 2 (iRhom1/2 or Rhbdf1/2). Most studies to date have focused on overexpressed iRhom1 and -2, so only little is known about the properties of the endogenous proteins. Here, we show that endogenous iRhom1 and -2 can be cell surface?biotinylated on mouse embryonic fibroblasts (mEFs), revealing that endogenous iRhom1 and -2 proteins are present on the cell surface and that iRhom2 also is present on the surface of lipopolysaccharide-stimulated primary bone marrow?derived macrophages. Interestingly, very little, if any, iRhom2 was detectable in mEFs or bone marrow?derived macrophages lacking ADAM17, suggesting that iRhom2 is stabilized by ADAM17. By contrast, the levels of iRhom1 were slightly increased in the absence of ADAM17 in mEFs, indicating that its stability does not depend on ADAM17. These findings support a model in which iRhom2 and ADAM17 are obligate binding partners and indicate that iRhom2 stability requires the presence of ADAM17, whereas iRhom1 is stable in the absence of ADAM17.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Adam ; Transmembrane Domain ; Protein Stability ; Myeloid Cell ; Metalloprotease ; Adam17 ; Rhbdf1 ; Inactive Rhomboid 1 ; Irhom1 ; Rhbdf2 ; Inactive Rhomboid 2 ; Irhom2 ; Sting ; Stimulator Of Interferon Genes ; Cell-surface Enzyme ; Membrane Protein; Necrosis-factor-alpha; Egf Receptor; Intracellular Maturation; Disintegrin; Metalloproteinase; Activation; Inflammation; Trafficking; Degradation; Secretion
ISSN (print) / ISBN
0021-9258
e-ISSN
1083-351X
Quellenangaben
Volume: 295,
Issue: 13,
Pages: 4350-4358
Publisher
American Society for Biochemistry and Molecular Biology
Publishing Place
11200 Rockville Pike, Suite 302, Rockville, Md, United States
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
CF Monoclonal Antibodies (CF-MAB)