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Wietrzynski, W. ; Schaffer, M.* ; Tegunov, D.* ; Albert, S.* ; Kanazawa, A.* ; Plitzko, J.M.* ; Baumeister, W.* ; Engel, B.D.

Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision.

eLife 9:e53740 (2020)
Postprint DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid network organizes these protein complexes to finely tune the photosynthetic reactions. Previously, we used in situ cryo-electron tomography to reveal the native architecture of thylakoid membranes (Engel et al., 2015). Here, we leverage technical advances to resolve the individual protein complexes within these membranes. Combined with a new method to visualize membrane surface topology, we map the molecular landscapes of thylakoid membranes inside green algae cells. Our tomograms provide insights into the molecular forces that drive thylakoid stacking and reveal that photosystems I and II are strictly segregated at the borders between appressed and non-appressed membrane domains. This new approach to charting thylakoid topology lays the foundation for dissecting photosynthetic regulation at the level of single protein complexes within the cell.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Chlamydomonas Reinhardtii ; Chloroplast ; Electron Microscopy ; In Situ ; Membranogram ; Molecular Biophysics ; Photosynthesis ; Plant Biology ; Structural Biology ; Thylakoid; Photosystem-ii; Chloroplast Membranes; Wild-type; Spatial-organization; Cytochrome B(6)f; Grana Margins; Higher-plants; Arrangement; Complexes; Dynamics
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2050-084X
e-ISSN 2050-084X
Journal eLife
Quellenangaben Volume: 9, Issue: , Pages: , Article Number: e53740 Supplement: ,
Publisher eLife Sciences Publications
Publishing Place Sheraton House, Castle Park, Cambridge, Cb3 0ax, England
Reviewing status Peer reviewed
Institute(s) Helmholtz Pioneer Campus (HPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Pioneer Campus
PSP Element(s) G-510008-001
DOI 10.7554/eLife.53740
WOS ID WOS:000527660000001
Scopus ID 85083477973
PubMed ID 32297859
Erfassungsdatum 2020-04-22
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