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Open Access Green as soon as Postprint is submitted to ZB.
Conditional gene trapping using the FLEx system.
Methods Mol. Biol. 435, 127-138 (2008)
The knowledge about the complete genome sequences of mouse, human, and other organisms is only the first step toward the functional annotation of all genes. It facilitates the recognition of sequence conservation, which helps to distinguish between important and not important and also coding from noncoding sequence. Nevertheless, approximately only 50% of all mouse genes have been entirely annotated to date. In the postgenomic era, large-scale projects have been initiated to describe also the expression (Emap, Eurexpress) and the function (International Gene Trap Consortium, Eucomm, Norcomm, Komp) of all mouse genes. By building up on these resources, the average amount of time starting from a gene-coding sequence to finally studying its function in a living organism or embryo, has shortened significantly within the last decade. Several recent developments, namely, in bioinformatics and gene synthesis but also in targeted and random mutagenesis have contributed to the current status. This chapter will highlight the milestones that have been undertaken in order to saturate the mouse genome with gene trap mutations. We have no intention to cover the entire field but will instead focus on most recent vectors and protocols, which have turned out to be most useful in order to promote the technology. Therefore, we apologize upfront to the many studies that could not be mentioned here solely owing to space limitations but which nevertheless made significant contributions to our current understanding. This chapter will finally provide guidance on possible uses of conditional gene trap alleles as well as detailed protocols for the application of this recent technology.
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Publication type
Article: Journal article
Document type
Scientific Article
Editors
Davis, G.D.* ; Kayser, K.J.*
Keywords
Conditional; gene trap; mutagenesis; Cre; Flpe; recombinase; FlEx; inversior
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Conference Title
Chromosomal Mutagenesis
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 435,
Pages: 127-138
Publisher
Springer
Publishing Place
Berlin [u.a.]
Reviewing status
Peer reviewed
Institute(s)
Institute of Developmental Genetics (IDG)