PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Huber, J.E.* ; Ahlfeld, J. ; Scheck, M.K.* ; Zaucha, M.* ; Witter, K.* ; Lehmann, L.* ; Karimzadeh, H. ; Pritsch, M.* ; Hoelscher, M.* ; von Sonnenburg, F.* ; Dick, A.* ; Barba-Spaeth, G.* ; Krug, A.B.* ; Rothenfußer, S. ; Baumjohann, D.*

Dynamic changes in circulating T follicular helper cell composition predict neutralising antibody responses after yellow fever vaccination.

Clin. Transl. Immunology 9:e1129 (2020)
Publ. Version/Full Text Research data DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Objectives T follicular helper (Tfh) cells are the principal T helper cell subset that provides help to B cells for potent antibody responses against various pathogens. In this study, we took advantage of the live-attenuated yellow fever virus (YFV) vaccine strain, YF-17D, as a model system for studying human antiviral immune responses in vivo following exposure to an acute primary virus challenge under safe and highly controlled conditions, to comprehensively analyse the dynamics of circulating Tfh (cTfh) cells.Methods We tracked and analysed the response of cTfh and other T and B cell subsets in peripheral blood of healthy volunteers by flow cytometry over the course of 4 weeks after YF-17D vaccination.Results Using surface staining of cell activation markers to track YFV-specific T cells, we found increasing cTfh cell frequencies starting at day 3 and peaking around 2 weeks after YF-17D vaccination. This kinetic was confirmed in a subgroup of donors using MHC multimer staining for four known MHC class II epitopes of YF-17D. The subset composition of cTfh cells changed dynamically during the course of the immune response and was dominated by the cTfh1-polarised subpopulation. Importantly, frequencies of cTfh1 cells correlated with the strength of the neutralising antibody response, whereas frequencies of cTfh17 cells were inversely correlated.Conclusion In summary, we describe detailed cTfh kinetics during YF-17D vaccination. Our results suggest that cTfh expansion and polarisation can serve as a prognostic marker for vaccine success. These insights may be leveraged in the future to improve current vaccine design and strategies.
Impact Factor
Scopus SNIP
Scopus
Cited By
Altmetric
6.464
1.689
20
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Neutralising Antibodies ; T Follicular Helper (tfh) Cells ; Vaccination ; Viral Infection ; Yellow Fever ; Yf-17d; Virus 17d; Effector; Immunity; Biology; Blood
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2050-0068
e-ISSN 2050-0068
Journal Clinical and translational immunology
Quellenangaben Volume: 9, Issue: 5, Pages: , Article Number: e1129 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Unit for Clinical Pharmacology (KKG-EKLiP)
Institute of Virology (VIRO)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Immune Response and Infection
PSP Element(s) G-522100-001
G-502700-003
DOI 10.1002/cti2.1129
WOS ID WOS:000537716300005
Scopus ID 85085279616
PubMed ID 32419947
Erfassungsdatum 2020-05-20
[➜Report correction]