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Oncogenic linear collagen VI of invasive breast cancer is induced by CCL5.
J. Clin. Med. 9:991 (2020)
The triple-negative breast tumor boundary is made of aligned, linear collagen. The pro-oncogenic impact of linear collagen is well established; however, its mechanism of formation is unknown. An in vitro analogue of the tumor border is created by a co-culture of MDA-MB-231 cells, adipose derived stem cells, and dermal fibroblasts. Decellularization of this co-culture after seven days reveals an extracellular matrix that is linear in fashion, high in pro-oncogenic collagen type VI, and able to promote invasion of reseeded cells. Further investigation revealed linear collagen VI is produced by fibroblasts in response to a paracrine co-culture of adipose derived stem cells and MDA-MB-231, which together secrete high levels of the chemokine CCL5. The addition of monoclonal antibody against CCL5 to the co-culture results in an unorganized matrix with dramatically decreased collagen VI. Importantly, reseeded cells do not exhibit pro-oncogenic behavior. These data illustrate a cellular mechanism, which creates linear extracellular matrix (ECM) in vitro, and highlight a potential role of CCL5 for building striated tumor collagen in vivo.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Triple Negative Breast Cancer ; Linear ; Border ; Collagen Vi ; Ccl5 ; Invasion ; Metastatic ; Adipose Derived Stem Cell ; Extracellular Matrix ; Decellularization; Label-free; Cells
ISSN (print) / ISBN
2077-0383
e-ISSN
2077-0383
Journal
Journal of Clinical Medicine
Quellenangaben
Volume: 9,
Issue: 4,
Article Number: 991
Publisher
MDPI
Publishing Place
Basel
Non-patent literature
Publications
Reviewing status
Peer reviewed