PuSH - Publication Server of Helmholtz Zentrum München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Weigand, M.* ; Degroote, R.L.* ; Amann, B.* ; Renner, S.* ; Wolf, E.* ; Hauck, S.M. ; Deeg, C.A.*

Proteome profile of neutrophils from a transgenic diabetic pig model shows distinct changes.

J. Proteomics 224:103843 (2020)
Postprint DOI PMC
Open Access Green
INSC94Y transgenic pigs develop a stable diabetic phenotype early after birth and therefore allow studying the influence of hyperglycemia on primary immune cells in an early stage of diabetes mellitus in vivo. Since immune response is altered in diabetes mellitus, with deviant neutrophil function discussed as one of the possible causes in humans and mouse models, we investigated these immune cells in INSC94Y transgenic pigs and wild type controls at protein level. A total of 2371 proteins were quantified by label-free LC-MS/MS. Subsequent differential proteome analysis of transgenic animals and controls revealed clear differences in protein abundances, indicating a deviant behavior of granulocytes in the diabetic state. Interestingly, abundance of myosin regulatory light chain 9 (MLC-2C) was increased 5-fold in cells of diabetic pigs. MLC-2C directly affects cell contractility by regulating myosin ATPase activity, can act as transcription factor and was also associated with inflammation. It might contribute to impaired neutrophil cell adhesion, migration and phagocytosis.Our study provides novel insights into proteome changes in neutrophils from a large animal model for permanent neonatal diabetes mellitus and points to dysregulation of neutrophil function even in an early stage of this disease.Data are available via ProteomeXchange with identifier PXD017274.Significance: Our studies provide novel basic information about the neutrophil proteome of pigs and contribute to a better understanding of molecular mechanisms involved in altered immune cell function in an early stage diabetes. We demonstrate proteins that are dysregulated in neutrophils from a transgenic diabetic pig and have not been described in this context so far. The data presented here are highly relevant for veterinary medicine and have translational quality for diabetes in humans.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
3.509
1.039
5
6
Tags
Annotations
Special Publikation
Hide on homepage

Edit extra information
Edit own tags
Private
Edit own annotation
Private
Hide on publication lists
on hompage
Mark as special
publikation
Publication type Article: Journal article
Document type Scientific Article
Keywords Quantitative Label-free Lc-ms/ms ; Diabetes Mellitus ; Neutrophil ; Granulocyte ; Transgenic Pig ; Myosin Regulatory Light Chain 9 (mlc-2c); Activation; Cells; Identification; Expression; Increases; Proteins; Alpha
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 1874-3919
e-ISSN 1876-7737
Journal Journal of Proteomics
Quellenangaben Volume: 224, Issue: , Pages: , Article Number: 103843 Supplement: ,
Publisher Elsevier
Publishing Place Radarweg 29, 1043 Nx Amsterdam, Netherlands
Reviewing status Peer reviewed
Institute(s) CF Metabolomics & Proteomics (CF-MPC)
POF-Topic(s) 30203 - Molecular Targets and Therapies
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505700-001
A-630700-001
DOI 10.1016/j.jprot.2020.103843
WOS ID WOS:000540896500008
Scopus ID 85085597170
PubMed ID 32470542
Erfassungsdatum 2020-06-17
[➜Report correction]