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Generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex.
Nat. Protoc. 6, 214-228 (2011)
Instructing glial cells to generate neurons may prove a strategy to replace neurons that have degenerated. Here we describe a robust protocol for the efficient in vitro conversion of postnatal astroglia from the murine cerebral cortex into functional, synapse-forming neurons. This protocol involves two steps: (i) expansion of astroglial cells (7 days) and (ii) astroglia-to-neuron conversion induced by persistent and strong retroviral expression of Neurogenin2 or Mash1 and/or Dlx2 for generation of glutamatergic or GABAergic neurons, respectively (7-21 days for different degrees of maturity). Our protocol of astroglia-to-neuron conversion by a single neurogenic transcription factor provides a stringent experimental system to study the specification of a selective neuronal subtype, thus offering an alternative to the use of embryonic or neural stem cells. Moreover it can be a useful model for studies of lineage conversion from non-neuronal cells with potential for brain regenerative medicine.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Cell fate; Glutamatergic; GABAergic; Neurogenin2; Mash1; Dlx2; Transcription factor
Language
english
Publication Year
2011
HGF-reported in Year
2011
ISSN (print) / ISBN
1754-2189
e-ISSN
1750-2799
Journal
Nature Protocols
Quellenangaben
Volume: 6,
Issue: 2,
Pages: 214-228
Publisher
Nature Publishing Group
Reviewing status
Peer reviewed
POF-Topic(s)
30204 - Cell Programming and Repair
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Research field(s)
Stem Cell and Neuroscience
PSP Element(s)
G-500800-001
G-501200-001
G-501200-001
PubMed ID
21293461
Scopus ID
79551663560
Erfassungsdatum
2011-04-13