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Mishra, K. ; Stankevych, M. ; Fuenzalida Werner, J.P. ; Grassmann, S.* ; Gujrati, V. ; Huang, Y. ; Klemm, U. ; Buchholz, V.R.* ; Ntziachristos, V. ; Stiel, A.-C.

Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.

Sci. Adv. 6:eaaz6293 (2020)
Publ. Version/Full Text Research data DOI PMC
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We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Photoacoustic Tomography; Bacteriophytochrome
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 2375-2548
e-ISSN 2375-2548
Journal Science Advances
Quellenangaben Volume: 6, Issue: 24, Pages: , Article Number: eaaz6293 Supplement: ,
Publisher American Association for the Advancement of Science (AAAS)
Publishing Place Washington, DC [u.a.]
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30205 - Bioengineering and Digital Health
Research field(s) Enabling and Novel Technologies
PSP Element(s) G-505591-004
G-505500-001
DOI 10.1126/sciadv.aaz6293
WOS ID WOS:000542291800018
Scopus ID 85086501699
PubMed ID 32582850
Erfassungsdatum 2020-06-23
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