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Burton, A. ; Brochard, V.* ; Galan, C.* ; Ruiz-Morales, E.R.* ; Rovira, Q.* ; Rodriguez-Terrones, D.* ; Kruse, K.* ; Le Gras, S.* ; Udayakumar, V.S.* ; Chin, H.G.* ; Eid, A. ; Liu, X.* ; Wang, C.* ; Gao, S.* ; Pradhan, S.* ; Vaquerizas, J.M.* ; Beaujean, N.* ; Jenuwein, T.* ; Torres-Padilla, M.E.

Heterochromatin establishment during early mammalian development is regulated by pericentromeric RNA and characterized by non-repressive H3K9me3.

Nat. Cell Biol. 22, 767-778 (2020)
Postprint DOI PMC
Open Access Green
Following fertilization in mammals, the gametes are reprogrammed to create a totipotent zygote, a process that involves de novo establishment of chromatin domains. A major feature occurring during preimplantation development is the dramatic remodelling of constitutive heterochromatin, although the functional relevance of this is unknown. Here, we show that heterochromatin establishment relies on the stepwise expression and regulated activity of SUV39H enzymes. Enforcing precocious acquisition of constitutive heterochromatin results in compromised development and epigenetic reprogramming, which demonstrates that heterochromatin remodelling is essential for natural reprogramming at fertilization. We find that de novo H3K9 trimethylation (H3K9me3) in the paternal pronucleus after fertilization is catalysed by SUV39H2 and that pericentromeric RNAs inhibit SUV39H2 activity and reduce H3K9me3. De novo H3K9me3 is initially non-repressive for gene expression, but instead bookmarks promoters for compaction. Overall, we uncover the functional importance for the restricted transmission of constitutive heterochromatin during reprogramming and a non-repressive role for H3K9me3.Burton et al. show that H3K9me3 deposition catalysed by SUV39H2 and regulated by pericentromeric RNAs in the mouse paternal pronucleus does not suppress gene expression, but bookmarks promoters for compaction.
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Publication type Article: Journal article
Document type Scientific Article
Keywords H3 Lysine-9 Methylation; Histone Modification; Nuclear Transfer; Dna Methylation; Gene-expression; Read Alignment; Cloned Embryos; Stem-cells; Chromatin; Seq
Language english
Publication Year 2020
HGF-reported in Year 2020
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Journal Nature Cell Biology
Quellenangaben Volume: 22, Issue: 7, Pages: 767-778 Article Number: , Supplement: ,
Publisher Nature Publishing Group
Publishing Place Macmillan Building, 4 Crinan St, London N1 9xw, England
Reviewing status Peer reviewed
Institute(s) Institute of Epigenetics and Stem Cells (IES)
POF-Topic(s) 30204 - Cell Programming and Repair
Research field(s) Stem Cell and Neuroscience
PSP Element(s) G-506200-001
DOI 10.1038/s41556-020-0536-6
WOS ID WOS:000544160700001
Scopus ID 85087022156
PubMed ID 32601371
Erfassungsdatum 2020-07-16
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