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Benedetti, E. ; Gerstner, N. ; Pučić-Baković, M.* ; Keser, T.* ; Reiding, K.R.* ; Ruhaak, L.R.* ; Štambuk, T.* ; Selman, M.H.J.* ; Rudan, I.* ; Polašek, O.* ; Hayward, C.* ; Beekman, M.* ; Slagboom, E.* ; Wuhrer, M.* ; Dunlop, M.G.* ; Lauc, G.* ; Krumsiek, J.

Systematic evaluation of normalization methods for glycomics data based on performance of network inference.

Metabolites 10:271 (2020)

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	Open Access Gold

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Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions. The removal of this non-biological signal from the data is referred to as normalization. Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far. In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach. It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion. Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway. The method therefore exploits a biological measure of goodness. We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography – ElectroSpray Ionization-Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization – Furier Transform Ion Cyclotron Resonance – Mass Spectrometry (MALDI-FTICR-MS). Based on our results, we recommend normalizing glycan data using the ‘Probabilistic Quotient’ method followed by log-transformation, irrespective of the measurement platform. This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Data Normalization ; Gaussian Graphical Models ; Glycomics; Compositional Data-analysis; Glycosylation; Allotypes; Discovery; Model

ISSN (print) / ISBN 2218-1989

e-ISSN 2218-1989

Journal Metabolites

Quellenangaben Volume: 10, Issue: 7, Article Number: 271

Publisher MDPI

Publishing Place St Alban-anlage 66, Ch-4052 Basel, Switzerland

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) Institute of Computational Biology (ICB)