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Bund, D. ; Buhmann, R. ; Gökmen, F. ; Kremser, A.* ; Dreyssig, J.* ; Kolb, H.-J. ; Schmetzer, H.M.

Canine-DCs using different serum-free methods as an approach to provide an animal-model for immunotherapeutic strategies.

Cell. Immunol. 263, 88-98 (2010)

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Open Access Green as soon as Postprint is submitted to ZB.

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Animal-models are the basis of DC-based human immunotherapies. We describe the standardization of a canine-DC-generation protocol using different cytokines and characterize the quality and functional repertoire of the obtained canine-DCs. DCs were generated from healthy dog-PBMCs under serum-free and serum-containing conditions. DC-quality and -quantity was determined by FACS studying the expression-profiles of DC-/costimulatory- and maturation-antigens before/after culture with canine and human monoclonal-antibodies (cmabs/hmabs). Individual DCAgs-(DC-antigens)-expression-profiles were found before and after culture depending on the agents' mode-of-action. With at least one of three serum-free methods (Ca-Ionophore, Picibanil, Cytokines) sufficient DC-amounts were generated. So, canine-DCs can be regularly generated under serum-free conditions and hmabs additionally to cmabs qualify for staining/quantification of canine-cells/DCs. The canine-DCs were functional, shown by T-cell-activation, -proliferation and antigen-specific CTL-responses. In summary, successful, quantitative DC-generation is possible with serum-free methods. DC-based T-cell-vaccination-strategies evaluated for e.g. AML-patients can be tested in the dog and estimated in clinical studies for DC-vaccination-strategies.

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Publication type Article: Journal article

Document type Scientific Article

Corresponding Author

Keywords Dendritic cells; Canine model; Immunotherapy; Serum-free culture

ISSN (print) / ISBN 0008-8749

e-ISSN 0008-8749

Journal Cellular Immunology

Quellenangaben Volume: 263, Issue: 1, Pages: 88-98

Publisher Elsevier

Non-patent literature Publications

Reviewing status Peer reviewed

Institute(s) CCG Hematopoetic Cell Transplants (IMI-KHZ)