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Solid state NMR assignments of a human λ-III immunoglobulin light chain amyloid fibril.
Biomol. NMR Assign. 15, 9-16 (2021)
The aggregation of antibody light chains is linked to systemic light chain (AL) amyloidosis, a disease where amyloid deposits frequently affect the heart and the kidney. We here investigate fibrils from the lambda-III FOR005 light chain (LC), which is derived from an AL-patient with severe cardiac involvement. In FOR005, five residues are mutated with respect to its closest germline gene segment IGLV3-19 and IGLJ3. All mutations are located close to the complementarity determining regions (CDRs). The sequence segments responsible for the fibril formation are not yet known. We use fibrils extracted from the heart of this particular amyloidosis patient as seeds to prepare fibrils for solid-state NMR. We show that the seeds induce the formation of a specific fibril structure from the biochemically produced protein. We have assigned the fibril core region of the FOR005-derived fibrils and characterized the secondary structure propensity of the observed amino acids. As the primary structure of the aggregated patient protein is different for every AL patient, it is important to study, analyze and report a greater number of light chain sequences associated with AL amyloidosis.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Al Amyloidosis ; Variable Light Chain Fibrils ; Solid State Nmr; Domain; C-13
Language
english
Publication Year
2021
Prepublished in Year
2020
HGF-reported in Year
2020
ISSN (print) / ISBN
1874-2718
e-ISSN
1874-270X
Journal
Biomolecular NMR Assignments
Quellenangaben
Volume: 15,
Issue: 1,
Pages: 9-16
Publisher
Springer
Publishing Place
Van Godewijckstraat 30, 3311 Gz Dordrecht, Netherlands
Reviewing status
Peer reviewed
Institute(s)
Institute of Structural Biology (STB)
POF-Topic(s)
30203 - Molecular Targets and Therapies
Research field(s)
Enabling and Novel Technologies
PSP Element(s)
G-503090-001
Grants
Projekt DEAL
WOS ID
WOS:000570844000001
Scopus ID
85091010918
Scopus ID
32946005
PubMed ID
32946005
Erfassungsdatum
2020-11-02